SerRed Nucleic Acid Gel Stain, 10,000× (Water-soluble)

SerRed Nucleic Acid Gel Stain 10,000× – 100µl

General Information

• Format: 10,000× concentrate, water-soluble
• Ethidium bromide (EtBr) replacement: same spectral characteristics as EtBr, so it works with existing EtBr imaging systems
• Higher sensitivity than EtBr, no destaining needed
• Described by the supplier as non-mutagenic

Staining Methods

• In-gel: add 5 µl per 50 ml of agarose gel and mix. It can go straight into the hot gel solution, or be mixed with buffer and agarose before heating.
• Post-staining: dilute 1:10,000 in 1× TAE or TBE (5 µl per 50 ml). Soak agarose gels 10 minutes in stain pre-heated to about 70°C, or 30 minutes at room temperature on a shaker. Soak acrylamide gels 30 to 60 minutes or longer.
• Staining solution can be reused about 3 times. Store it at room temperature away from light.

Typical Uses

• Staining double-stranded DNA in agarose and acrylamide gels
• Single-stranded DNA and RNA can also be stained, at lower sensitivity

Note: DNA fragments with sticky ends may show some smearing. This is a property of the dye.

Store and transport at room temperature, protected from light. Do not refrigerate.  If a precipitate forms, heat to 45–50°C for 2 minutes and shake to dissolve; this does not affect performance.