50× TAE Buffer

Tris-Acetate-EDTA (TAE) Buffer 50× – 500ml

General Information

• Format: 50× concentrate, dilute before use
• pH (1× working solution): 8.0 – 8.3 @ 25°C

Composition (50× concentrate)

• Tris-acetate: 2 M
• EDTA: 0.1 M

Composition (1× working solution)

• Tris-acetate: 40 mM
• EDTA: 2 mM

Preparation

Dilute 50-fold with distilled or ultrapure water before use.

Typical Uses

• Agarose gel electrophoresis of DNA
• Electrophoresis where DNA fragments will be recovered from the gel

Store and transport at room temperature. If a precipitate forms, warm to 37°C until dissolved; this does not affect performance.

For research use only.