10× TBST Buffer Solution

Tris-Buffered Saline with Tween-20 (TBST) 10× – 1L

General Information

• Format: 10× concentrate, dilute before use
• pH (1× working solution): 7.2 – 7.6 @ 25°C

Composition (10× concentrate)

• Tris: 100 mM
• Sodium Chloride (NaCl): 1.5 M
• Tween-20: 0.5%

Composition (1× working solution)

• Tris: 10 mM
• Sodium Chloride (NaCl): 150 mM
• Tween-20: 0.05%

Preparation

Mix 100 mL of concentrate with 900 mL of deionised or distilled water to make 1 L of 1× TBST.

Typical Uses

• Western blot membrane washing
• Blocking and antibody dilution for immunofluorescence and IHC
• Wash steps after antibody incubation

Store and transport at room temperature. Store diluted 1× solution at 4°C and use within one month. Crystals may form in the concentrate; warm to 37°C until dissolved.

For research use only.