SerBlue Nucleic Acid Gel Stain, 10,000× (Water-soluble)

Ready-to-use 10,000× concentrate for fast, sensitive visualization of dsDNA/ssDNA/RNA in agarose gels. Safer handling than ethidium bromide; image with blue-light or UV transilluminators. Use in-gel (1× final) or as a post-stain – no destain required. The dye can be excited by a 488 nm laser and directly observed with a blue light Gel cutter or a blue light scanner.

Lead time: 2 weeks approx.

Pack contents:

• 1 × 500 µL SerBlue (10,000×), water-soluble.

Key benefits:

• Blue-light compatible to help preserve DNA integrity for downstream cloning. 
• High sensitivity with low background; clear bands without destaining. 
• Flexible workflow: in-gel or post-stain; thermally stable for addition to hot agarose.

Typical use:

In-gel staining (gel dyeing):

1. Prepare the gel by adding 5 µL SerBlue (10,000×) per 50 mL molten agarose. SerBlue is thermally stable – add directly to hot agarose, or premix SerBlue with electrophoresis buffer + agarose powder and heat.
2. Pour, allow to solidify, load samples, and run electrophoresis as usual.
3. Image under blue light (UV can be used but bands will appear dimmer).

Post-stain (soak dyeing):

1. Run electrophoresis as usual.
2. Make a 3× staining solution by diluting the 10,000× stock ~1:3300 in 0.1 M NaCl (e.g., 15 µL SerBlue in 50 mL).
3. Submerge the gel in sufficient 3× solution and incubate 30 min at room temperature, protected from light. For polyacrylamide gels, stain 30–60 min (longer for higher % acrylamide).
4. Image under blue light.

Tip: the post-stain solution can be reused ~3×; store used solution protected from light.