SKU: G3332-50

SweScript RT II First-Strand cDNA Synthesis Kit

Available on Backorder

Categories: SKU: G3332-50

Complete two-step RT kit for fast, efficient first-strand cDNA from mRNA or total RNA. Includes primers and all core reagents. Just add RNA.

Lead time: 2 weeks approx.

Contents:

  • SweScript RT II Enzyme Mix (with RNase inhibitor)
  • 5× reaction buffer (with dNTPs & Mg²⁺)
  • Oligo(dT)18 primer
  • Random Hexamer primer
  • nuclease-free water

Key benefits:

  • Fast RT — cDNA synthesis can be completed in ~5 minutes
  • High-temperature tolerance (42–65 °C) for structured/GC-rich RNA
  • No RNase H activity — preserves RNA during RT
  • cDNA is ready for PCR/qPCR downstream

Typical use: 20 µL reaction; RT at 50–55 °C for 5–15 min (mix/type dependent). Quick start at 25 °C for 5 min if using Random Hexamers. Full protocol in manual.

Ships cold; store at −20 °C.

50 reactions per pack

 

 

Application

Reverse Transcription (cDNA)

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