SKU: G3332-50
SweScript RT II First-Strand cDNA Synthesis Kit
Available on Backorder
Categories: PCR & qPCR KitsSKU: G3332-50
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Complete two-step RT kit for fast, efficient first-strand cDNA from mRNA or total RNA. Includes primers and all core reagents. Just add RNA.
Lead time: 2 weeks approx.
Contents:
- SweScript RT II Enzyme Mix (with RNase inhibitor)
- 5× reaction buffer (with dNTPs & Mg²⁺)
- Oligo(dT)18 primer
- Random Hexamer primer
- nuclease-free water
Key benefits:
- Fast RT — cDNA synthesis can be completed in ~5 minutes
- High-temperature tolerance (42–65 °C) for structured/GC-rich RNA
- No RNase H activity — preserves RNA during RT
- cDNA is ready for PCR/qPCR downstream
Typical use: 20 µL reaction; RT at 50–55 °C for 5–15 min (mix/type dependent). Quick start at 25 °C for 5 min if using Random Hexamers. Full protocol in manual.
Ships cold; store at −20 °C.
50 reactions per pack
Application | Reverse Transcription (cDNA) |
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