SweScript One-Step RT-PCR Kit

One-tube RT + PCR workflow for RNA targets using gene-specific primers—add RNA, primers, and water; no tube changes.

Lead time: 2 weeks approx.

Contents:

• SweScript One-Step RT Mix (RT + RNase inhibitor)
• 2× One-Step PCR Master Mix (polymerase, buffer, dNTPs, Mg²⁺)
• Nuclease-free water

Key benefits:

• Single-tube setup reduces hands-on time and contamination risk
• Optimized chemistry for specific, high-performance amplification (up to ~8 kb amplicons)
• Streamlined program with RT at 50–55 °C, then PCR cycling

Typical use: 50 µL reaction; RT 15–30 min @ 50 °C (55 °C for GC-rich), then PCR per primer Tm (guide in manual). Use gene-specific primers (not random/oligo-dT).

Ships cold; store at −20 °C.