One-Tube gDNA Removal + RT (for qPCR)

Two-step RT kit with built-in genomic DNA removal. Prepare high-quality cDNA for qPCR in a single, simple setup.

Lead time: 2 weeks approx.

Contents:

• 5× SweScript All-in-One SuperMix for qPCR (RT, RNase inhibitor, oligo(dT)18 + random primers, dNTPs, optimized buffer)
• gDNA Remover (thermosensitive DNase)
• 5× No-RT Control SuperMix (RT-free control)
• Nuclease-free water

Key benefits

• One-step gDNA cleanup during RT → cleaner Ct values
• Easy workflow — add RNA + reagents, then run RT; cDNA is ready for qPCR
• Works with mRNA or total RNA; flexible priming (oligo(dT) + random)
• Streamlined controls included (No-RT control)

Typical use: 20 µL reaction; RT program: 25 °C 5 min → 42 °C 15–30 min → 85 °C 5 s. For GC-rich/structured RNA, pre-heat RNA/water to 65 °C for 5 min, cool, then add reagents. Full protocol in manual.

Ships cold; store at −20 °C.