Human Rotavirus RT-qPCR Test Kit

A complete kit for the detection of Human Rotavirus

Lead time: 2 weeks approx.

qPCR is the fastest and most precise method to identify and quantify pathogens. The YouSeq kit for Human Rotavirus contains all components necessary for easy, accurate detection on any qPCR platform. The target gene for Human Rotavirus (NSP5 gene,NSP5 gene,NSP3 gene) has been demonstrated to have a distinctive sequence making it an ideal target for highly specific detection of the target.

Contents:

• Target Specific primer/probe
• Endogenous control primer/probe
• qPCR OneStep MasterMix
• Positive control template
• DNase/RNase free water
• Template resuspension buffer
• ROX passive reference

Technical Specifications:

• Target Gene(s):NSP5 gene,NSP5 gene,NSP3 gene
• Efficiency: 90-110%
• Sensitivity: <100 copy detection
• Run Time: Approx 1hr30 (dependent on instrumentation)
• Target Pathogen: FAM labelled
• Endogenous Control: VIC/HEX labelled

Materials Required But Not Included:

• RNA Extraction kit – This qPCR test kit will work well with high quality RNA derived from any extraction kit with minimal PCR inhibitors present. 
• qPCR instrument with minimum 2 colour detection (FAM and VIC/HEX).
• Pipettes, micro centrifuge tubes and general laboratory equipment.

100 reactions per kit